Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile assay for high-throughput gene expression profiling on universal array matrices.

Jian-Bing Fan1, Joanne M Yeakley, Marina Bibikova

  • 1Illumina, Inc., San Diego, California 92121, USA. jfan@illumina.com

Genome Research
|May 5, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic reconstruction of upland cotton domestication uncovers staged selection, gene flow, and flowering-time adaptation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

SpliceHarmonization: an integrated method for identifying RNA splicing events in therapeutics for splicing modulation.

Bioinformatics (Oxford, England)·2026
Same author

CRISPR genome editing in plants without tissue culture.

Trends in biotechnology·2026
Same author

Scaling up spatial transcriptomics for large-sized tissues: uncovering cellular-level tissue architecture beyond conventional platforms with iSCALE.

Nature methods·2025
Same author

Establishing clinically relevant dissolution specifications for prodrug bioequivalence risk assessment: Integration of a dissolution/permeation system with physiologically based biopharmaceutics modeling in abiraterone acetate.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same author

Enhance therapeutic efficacy of BiTE (HER2/CD3) for HER2- positive tumors through <i>in vivo</i> expression.

International journal of pharmaceutics: X·2025
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
See all related articles

A new gene expression profiling system enables sensitive and quantitative analysis of over 400 genes across 96 samples. This cDNA-mediated annealing, selection, extension and ligation (DASL) assay offers a flexible, high-throughput, and cost-effective solution for large-scale gene expression studies.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression profiling is crucial for understanding cellular function and disease.
  • Existing methods can be limited in throughput, sensitivity, or cost-effectiveness for large-scale studies.

Purpose of the Study:

  • To develop and validate a flexible, sensitive, and quantitative gene-expression profiling system.
  • To enable high-throughput analysis of hundreds of genes across thousands of samples.

Main Methods:

  • Utilized the cDNA-mediated annealing, selection, extension and ligation (DASL) assay.
  • Employed oligonucleotides with unique address sequences for hybridization to universal arrays.
  • Applied the assay to hormone-treated cell lines and laser-capture microdissected cancer tissues.

Related Experiment Videos

  • Automated the assay using a bead-based 96-array matrix system.

    • Main Results:

    • Successfully profiled gene expression for over 400 genes across 96 samples in parallel.
    • Obtained cell-specific gene expression profiles from various biological samples.
    • Demonstrated consistency between DASL assay results and quantitative real-time PCR (qRT-PCR).

    Conclusions:

    • The DASL assay provides a flexible, sensitive, and quantitative method for gene expression profiling.
    • The automated, high-throughput system is accurate, efficient, and cost-effective for large-scale gene expression analysis.
    • This technology facilitates comprehensive gene expression studies in diverse biological contexts.