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Characterization of bacteriophage BFK20 from Brevibacterium flavum
M Koptides1, I Barák, M Sisová
1Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Czech.
Journal of General Microbiology
|July 1, 1992
Abstract:
Bacteriophage BFK20 was isolated from a Brevibacterium flavum strain that had become contaminated during industrial fermentation. BFK20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. The genome of this bacteriophage consists of a linear double stranded DNA molecule of 44-45 kb with cohesive ends. The capsid of phage BFK20 contains nine polypeptides with molecular masses from 22.0-108.0 kDa. BFK20 DNA was used as a donor for fragments carrying promoters and transcription-terminators.