Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin spatial dynamics in RBL-2H3 mast cells.

Maria Psatha1, Anna Koffer, Muriel Erent

  • 1Department of Physiology, University College London, London, UK.

Cell Calcium
|May 6, 2004
PubMed
Summary

Calmodulin (CaM) localization in rat basophilic leukemia (RBL) cells reveals its association with the actin cytoskeleton and centrosome. Upon stimulation, CaM redistributes, potentially organizing granule transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-tailed class I myosins rely on tail-mediated phosphoinositide recognition for specific membrane recruitment.

Cell communication and signaling : CCS·2025
Same author

Leucine-rich α-2-glycoprotein 1 initiates the onset of diabetic retinopathy in mice.

Science translational medicine·2025
Same author

LRG1 Alters Pericyte Phenotype and Compromises Vascular Maturation.

Cells·2025
Same author

Leucine-Rich Alpha-2-Glycoprotein 1 Promotes Metastatic Colorectal Cancer Growth Through Human Epidermal Growth Factor Receptor 3 Signaling.

Gastroenterology·2024
Same author

The disruptive role of LRG1 on the vasculature and perivascular microenvironment.

Frontiers in cardiovascular medicine·2024
Same author

The first mitotic division of human embryos is highly error prone.

Nature communications·2022

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • Calmodulin (CaM) is a crucial calcium-binding protein involved in numerous cellular processes.
  • Mast cells, like rat basophilic leukemia (RBL) cells, play a key role in allergic responses and inflammation.
  • Understanding CaM dynamics in mast cells is essential for elucidating signaling pathways.

Purpose of the Study:

  • To investigate the localization and dynamics of calmodulin in living rat basophilic leukemia (RBL) cells.
  • To explore the role of calmodulin in cellular processes, including cell division and response to stimulation.
  • To characterize calmodulin's interaction with cytoskeletal components and intracellular organelles.

Main Methods:

  • Utilized a stable cell line of RBL cells expressing EGFP-tagged calmodulin (CaM-EGFP).

Related Experiment Videos

  • Employed fluorescence microscopy to visualize CaM localization in interphase, dividing, and stimulated cells.
  • Disrupted the actin cytoskeleton to assess CaM's association with actin.
  • Used lysotracker to track granule movement and its colocalization with CaM puncta.

    • Main Results:

    • In unstimulated RBL cells, CaM localized to the cell cortex and the centrosome.
    • Disruption of actin cytoskeleton affected cortical CaM localization, while central localization was linked to tubulin and the centrosome.
    • Cell stimulation led to CaM redistribution, with increased cortical fluorescence and the appearance of CaM-EGFP puncta in the cell interior.
    • These puncta colocalized with moving granules, suggesting a role for CaM in granule transport.

    Conclusions:

    • A significant portion of calmodulin in interphase RBL cells is associated with the actin cytoskeleton.
    • Calmodulin dynamics change upon cell stimulation, indicating its involvement in signaling pathways.
    • The study highlights the utility of the CaM-EGFP RBL cell model for studying calmodulin biology and mast cell function.