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-1 frameshifting at a CGA AAG hexanucleotide site is required for transposition of insertion sequence IS1222
Nina Mejlhede1, Patricia Licznar, Marie-Françoise Prère
1Laboratoire de Microbiologie et Génétique Moléculaire, UMR5100 Centre National de la Recherche Scientifique et Université Paul Sabatier, Toulouse 31062, France.
Journal of Bacteriology
|May 6, 2004
Abstract:
The discovery of programmed -1 frameshifting at the hexanucleotide shift site CGA_AAG, in addition to the classical X_XXY_YYZ heptanucleotide shift sequences, prompted a search for instances among eubacterial insertion sequence elements. IS1222 has a CGA_AAG shift site. A genetic analysis revealed that frameshifting at this site is required for transposition.