Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

First bacterial chalcone isomerase isolated from Eubacterium ramulus.

Claudia Herles1, Annett Braune, Michael Blaut

  • 1Abteilung Gastrointestinale Mikrobiologie, Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany.

Archives of Microbiology
|May 6, 2004
PubMed
Summary

Eubacterium ramulus possesses a novel bacterial chalcone isomerase enzyme. This enzyme converts flavanones like naringenin into chalcones, a key step in flavonoid degradation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Akkermansia muciniphila ameliorates Salmonella-induced colitis and intestinal fibrosis.

Mucosal immunology·2025
Same author

Flavonoid-converting capabilities of Clostridium butyricum.

Applied microbiology and biotechnology·2025
Same author

Genomics and physiology of <i>Catenibacillus</i>, human gut bacteria capable of polyphenol <i>C</i>-deglycosylation and flavonoid degradation.

Microbial genomics·2024
Same author

Optimization of flavanonols heterologous biosynthesis in <i>Streptomyces albidoflavus</i>, and generation of auronols.

Frontiers in microbiology·2024
Same author

Developmental metformin exposure does not rescue physiological impairments derived from early exposure to altered maternal metabolic state in offspring mice.

Molecular metabolism·2023
Same author

Fluorogenic substrates and pre-column derivatization for monitoring the activity of bile salt hydrolase from Clostridium perfringens.

Bioorganic chemistry·2023

Area of Science:

  • Microbiology
  • Biochemistry
  • Enzymology

Background:

  • Eubacterium ramulus, a human gut anaerobe, degrades various flavonoids.
  • Flavonoid degradation pathways often begin with isomerization of flavanones to chalcones.

Purpose of the Study:

  • To identify and characterize a chalcone isomerase from Eubacterium ramulus.
  • To investigate the enzyme's role in naringenin metabolism.

Main Methods:

  • Purification of chalcone isomerase from E. ramulus cell-free extracts.
  • Enzyme activity assays using various chalcone substrates.
  • Determination of enzyme molecular mass and subunit composition.

Main Results:

  • A bacterial chalcone isomerase was purified to homogeneity.

Related Experiment Videos

  • The enzyme exhibited activity on naringenin chalcone, isoliquiritigenin, and butein.
  • Molecular mass was estimated at 136/129 kDa (native) with a 30 kDa subunit.
  • N-bromosuccinimide, naringenin, and phloretin inhibited the enzyme.

    • Conclusions:

    • This is the first report of a bacterial chalcone isomerase.
    • The enzyme likely plays a role in the conversion of naringenin to its chalcone form.
    • Further research is needed to elucidate its precise physiological function.