Jove
Visualize
Contact Us

Related Experiment Videos

Gentamicin release from two-solution and powder-liquid poly(methyl methacrylate)-based bone cements by using novel pH

Imad K Merkhan1, Julie M Hasenwinkel, Jeremy L Gilbert

  • 1Department of Bioengineering and Neuroscience, 373 Link Hall, Syracuse University, Syracuse, New York 13244-5290, USA. ikmerkha@syr.edu

Journal of Biomedical Materials Research. Part A
|May 6, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrosion Damage in Retrieved CoCrMo/Ti-6Al-4V Modular Junctions in Femoral Revision Total Knee Components.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Metal Fatigue Fracture After Revision Total Knee Arthroplasty: A Retrieval Analysis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

In Service to the Journal of Biomedical Materials Research-Part B: Applied Biomaterials.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Optimization of lesser tuberosity osteotomy repair: a biomechanical assessment of suture tensioning, repair configuration, and type of suture.

JSES international·2026
Same author

In Vivo Corrosion of Acetabular Modular Tapers Reduces Local Corrosion Resistance: Near-Field Electrochemical Impedance Spectroscopy as an Indicator of the Type and Severity of Corrosion in Retrieved Hip Implants.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Electrocautery Alters the Near-Surface Microstructure, Phases, Composition, and Hardness of Ti-6Al-4V.

Journal of biomedical materials research. Part A·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

A novel pH technique accurately measures gentamicin release from bone cement. Palacos bone cement showed favorable gentamicin elution compared to two-solution cements, with mixing conditions impacting early release rates.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Analytical Chemistry

Background:

  • Gentamicin elution from bone cement is critical for preventing post-operative infections.
  • Accurate and efficient methods are needed to quantify gentamicin release kinetics.
  • Poly(methyl methacrylate) (PMMA)-based bone cements are widely used in orthopedic surgery.

Purpose of the Study:

  • To develop and validate a novel pH-based in vitro technique for measuring gentamicin release from bone cements.
  • To compare the gentamicin release profiles of Palacos bone cement with a two-solution bone cement.
  • To investigate the effect of mixing conditions on gentamicin elution.

Main Methods:

  • A novel pH technique was developed based on the linear relationship between gentamicin concentration and pH in aqueous solutions.

Related Experiment Videos

  • Continuous pH readings in phosphate-buffered saline (PBS) at 37°C were used to determine gentamicin release kinetics.
  • Gentamicin release was measured for Palacos R-40 and a custom two-solution bone cement under mixed and static conditions.

    • Main Results:

    • Palacos R-40 released 4.95 ± 0.22 wt% of gentamicin after 24 hours in PBS.
    • Palacos samples released 4.84 ± 0.27 mg, while a two-solution cement with equivalent gentamicin sulfate released 3.81 ± 0.52 mg.
    • A higher percentage of gentamicin release was observed from Palacos compared to the two-solution cement.

    Conclusions:

    • Monitoring pH is an effective, inexpensive, and direct in vitro technique for quantifying gentamicin release from PMMA-based bone cements.
    • Palacos bone cement demonstrates favorable gentamicin elution characteristics compared to the tested two-solution cement.
    • Mixing conditions significantly affected early-stage gentamicin release but not at the 24-hour mark.