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Related Experiment Videos

Microbiologic diagnostics at titanium implants.

Asa Leonhardt1, Christina Bergström, Ulf Lekholm

  • 1Department of Periodontology, Specialist Dental Clinic, Mölndal Hospital, SE 431 80 Mölndal, Sweden. asa.leonhardt@vgregion.se

Clinical Implant Dentistry and Related Research
|May 7, 2004
PubMed
Summary
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Microbiologic diagnosis of periimplant lesions is valuable. Combining DNA-DNA hybridization and culture techniques provides a comprehensive analysis of periimplant pathogens.

Area of Science:

  • Microbiology
  • Periodontology
  • Biotechnology

Background:

  • Periimplant lesions harbor periodontal pathogens and opportunistic species like Staphylococcus, enterics, and Candida.
  • Microbiologic diagnosis can guide treatment for periimplant lesions.

Purpose of the Study:

  • To evaluate the prevalence of putative pathogens in long-term cases.
  • To compare two different microbiologic diagnostic procedures.

Main Methods:

  • Plaque samples from teeth and implants of 15 subjects were analyzed.
  • 18 putative periimplant pathogens were detected using cultural methods and DNA-DNA hybridization.

Main Results:

  • DNA-DNA hybridization showed similar pathogen prevalence in teeth and implant samples.

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  • Culture techniques detected fewer positive individuals compared to checkerboard analysis at implant sites.
  • The two methods supplemented each other, with high specificity for key pathogens.

    • Conclusions:

    • A combination of DNA-DNA hybridization and culture protocols is recommended for comprehensive analysis of periimplant microbiota.
    • This combined approach yields the most complete outcome for diagnosing periimplant lesions.