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Structural and functional differences in two cyclic bacteriocins with the same sequences produced by lactobacilli.

Yasushi Kawai1, Yasuyuki Ishii, Kensuke Arakawa

  • 1Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. ykawai@bios.tohoku.ac.jp

Applied and Environmental Microbiology
|May 7, 2004
PubMed
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Two similar bacteriocins from Lactobacillus strains show different effects due to D-amino acid content. This difference impacts their action, potassium ion efflux, and structure, offering new insights into bacteriocin characteristics.

Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Lactobacillus gasseri LA39 and L. reuteri LA6 produce similar cyclic bacteriocins, gassericin A and reutericin 6.
  • Despite similar molecular weights and amino acid sequences, reutericin 6 exhibits a narrower inhibitory spectrum than gassericin A.

Purpose of the Study:

  • To investigate the differences in mode of action, potassium ion efflux, and secondary structures between gassericin A and reutericin 6.
  • To determine if D-amino acid content influences the distinct characteristics of these bacteriocins.

Main Methods:

  • Comparative analysis of bacteriocin activity against indicator strains.
  • Measurement of potassium ion efflux from cells and liposomes.
  • Circular dichroism spectroscopy to analyze secondary structures.

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  • D- and L-amino acid composition analysis.

    • Main Results:

    • Gassericin A inhibited L. reuteri LA6, while reutericin 6 did not inhibit L. gasseri LA39.
    • Both bacteriocins induced potassium ion efflux, but with different patterns and amounts.
    • Circular dichroism spectra indicated differences in secondary structures.
    • Gassericin A contained more D-alanine residues than reutericin 6.

    Conclusions:

    • Differences in D-alanine content likely account for the variations in bacteriocin activity, ion efflux, and structure.
    • This is the first report showing D-amino acid content influencing native bacteriocin characteristics from strains with identical structural genes.