Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel polyketide synthase from Nectria haematococca.

Stephane Graziani1, Christelle Vasnier, Marie-Josee Daboussi

  • 1Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France.

Applied and Environmental Microbiology
|May 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigation of a squaramide motif as a bioisostere of the amino-acid group of S-adenosyl-L-methionine and its functional impact on RNA methylation.

Communications chemistry·2025
Same author

sRNA-controlled iron sparing response in Staphylococci.

Nucleic acids research·2022
Same author

FDXR Mutations Cause Sensorial Neuropathies and Expand the Spectrum of Mitochondrial Fe-S-Synthesis Diseases.

American journal of human genetics·2017
Same author

Mutations in MDH2, Encoding a Krebs Cycle Enzyme, Cause Early-Onset Severe Encephalopathy.

American journal of human genetics·2016
Same author

Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy.

American journal of human genetics·2016
Same author

Reconstruction of ancestral chromosome architecture and gene repertoire reveals principles of genome evolution in a model yeast genus.

Genome research·2016

Researchers identified a novel polyketide synthase (PKS) gene, pksN, in Nectria haematococca. This gene is crucial for producing the red pigment found in the fungus's reproductive structures.

Area of Science:

  • Mycology
  • Biochemistry
  • Molecular Biology

Background:

  • Fungal secondary metabolites, including pigments, play roles in development and ecological interactions.
  • Polyketide synthases (PKS) are key enzymes in the biosynthesis of diverse secondary metabolites.
  • Nectria haematococca produces a red pigment in its perithecia, but the genetic basis is unknown.

Purpose of the Study:

  • To identify the gene responsible for the red perithecial pigment biosynthesis in Nectria haematococca.
  • To characterize the identified gene and its encoded protein product.
  • To elucidate the function of the gene in fungal development.

Main Methods:

  • Complementation of a pigment-deficient mutant strain.
  • Gene identification and sequencing.

Related Experiment Videos

  • Bioinformatic analysis of the deduced protein sequence.
  • Gene knockout (inactivation) and phenotypic analysis.

    • Main Results:

    • A novel polyketide synthase (PKS) gene, designated pksN, was identified in Nectria haematococca.
    • pksN encodes a large polypeptide (2,106 amino acids) with conserved type I PKS domains (beta-ketoacyl synthase, acyltransferase, acyl carrier proteins, thioesterase).
    • The pksN gene product clusters with other fungal WA-type PKSs involved in pigment and toxin biosynthesis.
    • Inactivation of pksN did not affect fungal growth, sporulation, or ascospore formation, but abolished red perithecial pigmentation.

    Conclusions:

    • pksN is essential for the biosynthesis of the red perithecial pigment in Nectria haematococca.
    • The identified PKS is a novel enzyme involved in a specific developmental function related to pigmentation.
    • This finding contributes to understanding fungal secondary metabolism and pigment production pathways.