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Related Experiment Videos

Methods for quantifying follicular numbers within the mouse ovary.

M Myers1, K L Britt, N G M Wreford

  • 1Department of Anatomy and Cell Biology, School of Biomedical Sciences, Monash University, Clayton, VIC, Australia.

Reproduction (Cambridge, England)
|May 7, 2004
PubMed
Summary

Accurate ovarian follicle counting is crucial for understanding female reproductive health. This study introduces unbiased stereological methods for precise quantification of mouse ovarian follicles, improving research reliability.

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Stereology

Background:

  • Accurate estimation of ovarian follicle numbers is vital for assessing folliculogenesis and reproductive function.
  • Previous methods exhibit significant variability (10-fold or greater), hindering reliable interpretation of ovarian studies.
  • Discrepancies in reported follicular counts complicate comparisons between experimental and control conditions.

Purpose of the Study:

  • To describe unbiased, assumption-free stereological methods for quantifying early and growing follicular populations in the mouse ovary.
  • To establish a reliable methodology for assessing ovarian function and facilitating inter-study comparisons.

Main Methods:

  • Employed a fractionator approach to sample defined fractions of histological sections from adult wild-type mouse ovaries.

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  • Utilized optical and physical disector methods for independent counting of primordial and primary follicles.
  • Applied fractionator/disector techniques, independent of follicular size or shape, for accurate quantification.

    • Main Results:

    • Estimated 1930 ± 286 (S.E.M.) and 2227 ± 101 primordial follicles per ovary at 70 and 100 days of age, respectively.
    • Estimated 137 ± 25 and 265 ± 32 primary follicles per ovary at 70 and 100 days of age, respectively.
    • Estimated 135 secondary and later follicles per ovary at 100 days of age, and approximately 500 remnants of zona pellucidae (indicating atresia).

    Conclusions:

    • The described stereological methods provide accurate and unbiased quantification of ovarian follicles at various developmental stages.
    • This quantitative approach enhances the understanding of follicular dynamics, growth regulation, and maturation processes.
    • The methodology facilitates improved comparability and reliability across different research studies in reproductive biology.