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Role of Ca2+ in induction of neurotransmitter-related gene expression by butyrate
Muhammad T Zia1, Bistra B Nankova, Kavitha Krishnan
1Division of Newborn Medicine Westchester Medical Center/Department of Pediatrics; Department of Biochemistry and Molecular Biology, The Regional Neonatal Center, New York Medical College-Westchester Medical Center, Valhalla, NY 10595, USA.
Abstract:
We examined the effect of butyrate on neurotransmitter-related gene expression and calcium homeostasis in PC12 cells. Pretreatment with Ca2+ chelators (EGTA or BAPTA-AM) attenuated the butyrate-triggered accumulation of TH and ppEnk mRNA indicating that Ca2+ plays a role in butyrate-induced regulation of neuronal genes. Butyrate alone did not alter intracellular Ca2+ levels as determined by Fura-PE3 fluorescence; however, pretreatment with butyrate (18-24 h) reduced the first Ca2+ peak and prevented the second sustained rise in [Ca2+]i as induced by nicotine or ryanodine. In contrast, butyrate had no effect on Ca2+ transients when added shortly before or during nicotine or ryanodine stimulation. These results suggest that chronic butyrate exposure can modulate cell responses by affecting intracellular Ca2+ signaling.
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