Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos
Gyulin Hwang1, Ferenc Müller, M Aziz Rahman
1Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK.
Marine Biotechnology (New York, N.Y.)
|May 7, 2004
Abstract:
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
