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Updated: Aug 24, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Hyperspectral imager, from ultraviolet to visible, with a KDP acousto-optic tunable filter
Neelam Gupta1, Vitaly Voloshinov
1U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783-1197, USA. ngupta@arl.army.mil
Abstract:
Hyperspectral imaging in the ultraviolet to visible spectral region has applications in astronomy, biology, chemistry, medical sciences, etc. A novel electronically tunable, random-wavelength access, compact, no-moving-parts, vibration-insensitive, computer-controlled hyperspectral imager operating from 220 to 480 nm with a spectral resolution of 160 cm(-1), e.g., 2 nm at 350 nm, has been developed by use of a KDP acousto-optic tunable filter (AOTF) with an enhanced CCD camera and a pair of crossed calcite Glan-Taylor polarizing prisms. The linear and angular apertures of the AOTF are 1.5 x 1.5 cm2 and 1.2 degrees, respectively. Imager setup and spectral imaging results as well as analyses and discussion of various factors affecting image quality are presented.
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