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Updated: Aug 24, 2026
Fibro-Adipogenic Progenitor Isolation, Expansion, and Differentiation from the Spiny Mouse Model
Published on: November 15, 2024
Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo
Toshiyuki Asaki1, Morichika Konishi, Ayumi Miyake
1Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo, Kyoto 606-8501, Japan.
Abstract:
The development of white adipose tissue (WAT) of Fgf10-/- mouse embryos was greatly impaired. Here, we examined the mechanism of Fgf10 action in adipogenesis in vivo. The proliferative activity in the WAT of Fgf10-/- embryos was greatly decreased. We also examined the expression of transcription factors, C/EBPbeta, C/EBPalpha and PPARgamma, that are important for adipogenesis. Although the expression of C/EBPbeta and PPARgamma in the WAT of Fgf10-/- embryos was greatly decreased, the expression of C/EBPalpha was essentially unchanged. Therefore, we examined their expression in the WAT of C/EBPalpha-/- embryos. Although the expression of C/EBPbeta and PPARgamma in the WAT was greatly decreased, the expression of Fgf10 was essentially unchanged. As these results in vivo appeared to be contradictory to a transcriptional cascade model in vitro that C/EBPbeta induces the expression of PPARgamma and C/EBPalpha reported, we also examined their expression in the WAT of wild type embryos at different developmental stages. The expression of Fgf10 and C/EBPalpha was followed by that of C/EBPbeta and PPARgamma. The present findings indicate that Fgf10 but not C/EBPalpha is required for the proliferation of preadipocytes. In contrast, both Fgf10 and C/EBPalpha acting synergistically in separate, parallel pathways are required for the differentiation. Unexpectedly, the transcriptional cascade of adipogenesis in vivo described here is distinct from the cascade in vitro previously reported.
Insights
Fibroblast growth factor 10 (Fgf10) is crucial for white adipose tissue (WAT) development and preadipocyte proliferation. Fgf10 and C/EBPalpha act synergistically in parallel pathways for adipocyte differentiation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Cell Biology
Background:
- White adipose tissue (WAT) development is essential for metabolic homeostasis.
- Fibroblast growth factor 10 (Fgf10) plays a role in embryonic development.
- Adipogenesis involves a complex network of transcription factors, including C/EBPbeta, C/EBPalpha, and PPARgamma.
Purpose of the Study:
- To elucidate the in vivo mechanism of Fgf10 action in adipogenesis.
- To investigate the role of Fgf10 in preadipocyte proliferation and differentiation.
- To clarify the relationship between Fgf10 and key adipogenic transcription factors.
Main Methods:
- Analysis of Fgf10 knockout (Fgf10-/-) mouse embryos.
- Examination of white adipose tissue (WAT) from Fgf10-/- and C/EBPalpha knockout (C/EBPalpha-/-) embryos.
- Assessment of cell proliferation and expression of adipogenic transcription factors (C/EBPbeta, C/EBPalpha, PPARgamma) at different developmental stages.
Main Results:
- Fgf10 deficiency significantly impaired WAT development and preadipocyte proliferation.
- Fgf10 knockout reduced C/EBPbeta and PPARgamma expression but not C/EBPalpha.
- Fgf10 and C/EBPalpha act synergistically in parallel pathways for adipocyte differentiation, distinct from in vitro models.
Conclusions:
- Fgf10 is essential for preadipocyte proliferation, while C/EBPalpha is not.
- Both Fgf10 and C/EBPalpha are required for adipocyte differentiation, acting via synergistic, parallel pathways.
- The in vivo transcriptional cascade of adipogenesis differs from previously reported in vitro models.
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