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Published on: July 9, 2014
Amplification of coccidioidal DNA in clinical specimens by PCR
Suzanne M Johnson1, Keira A Simmons, Demosthenes Pappagianis
1Department of Medical Microbiology and Immunology, School of Medicine, University of California-Davis, Davis, California 95616, USA. smjohnson@ucdavis.edu
Journal of Clinical Microbiology
|May 8, 2004
Abstract:
Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.
