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Related Experiment Videos

DNA macroarray for identification and typing of Staphylococcus aureus isolates.

Salim Trad1, Jeanine Allignet, Lionel Frangeul

  • 1Département Ecosystèmes et Epidémiologie des Maladies Infectieuses, Institut Pasteur, Paris, France.

Journal of Clinical Microbiology
|May 8, 2004
PubMed
Summary

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A novel DNA macroarray effectively identifies Staphylococcus aureus and types isolates based on gene content. This method offers superior discrimination compared to traditional typing, aiding in outbreak investigations and understanding virulence factors.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Staphylococcus aureus identification and typing are crucial for infection control and epidemiology.
  • Traditional methods like antibiograms and SmaI profiling have limitations in discriminating between closely related isolates.

Purpose of the Study:

  • To develop and evaluate a DNA macroarray for accurate Staphylococcus aureus species identification and isolate typing.
  • To assess the utility of gene content profiling for epidemiological studies and virulence factor analysis.

Main Methods:

  • A DNA macroarray with 465 intragenic amplicons targeting species-specific, antibiotic resistance, virulence, and mobile element genes was designed.
  • The macroarray was hybridized with DNA from 80 S. aureus clinical isolates previously characterized by antibiograms and SmaI patterns.

Related Experiment Videos

  • Gene content data was used to construct a dendrogram for isolate clustering and comparison with SmaI patterns.

    • Main Results:

    • The macroarray successfully identified S. aureus and clustered 80 isolates into 52 distinct groups based on gene content.
    • Gene content-based clustering provided better discrimination than SmaI analysis for certain endemic mecA(+) isolates.
    • The sdrD gene was significantly more prevalent in isolates from bone infections compared to nasal carriers, suggesting a role in invasive disease.

    Conclusions:

    • The developed DNA macroarray is a rapid and effective tool for S. aureus identification and typing.
    • Gene content profiling offers valuable insights into isolate characteristics, including virulence potential and epidemiological links.
    • This macroarray facilitates a deeper understanding of S. aureus epidemiology and pathogenesis.