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Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species
Caroline Massonet1, Johan Van Eldere, Mario Vaneechoutte
1Department of Immunology and Microbiology, Rega Institute, U.Z. Gasthuisberg, B-3000 Leuven, Belgium. caroline.massonet@med.kuleuven.ac.be
Abstract:
A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.
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