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Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR

Kyle T Beggs1, Ann R Holmes, Richard D Cannon

  • 1Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin 9001, New Zealand.

Journal of Clinical Microbiology
|May 8, 2004
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...