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Generation of transgenic mice
Guoping Feng1, Jing Lu, Jimmy Gross
1Department of Neurobiology, Duke University Medical Center, Durham, NC, USA.
Methods in Molecular Medicine
|May 8, 2004
Summary
Generating transgenic mice is crucial for understanding gene function and human disease implications. This chapter details essential protocols for creating these valuable research models.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Biomedical Research
Background:
- The post-genomic era focuses on gene product function and disease relevance.
- Transgenic mice are essential tools for studying gene function and regulation.
Purpose of the Study:
- To provide an overview and detailed protocols for generating transgenic mice.
- To highlight the diverse applications of transgenic mice in biomedical research.
Main Methods:
- Selection of regulatory elements for gene expression.
- Purification of DNA for microinjection.
- Pronuclear injection into fertilized eggs.
- Genotyping of resulting transgenic mice.
Main Results:
- Established protocols for generating transgenic mice.
- Demonstrated applications including gain-of-function, loss-of-function, domain mapping, regulatory element mapping, and cell labeling.
- Facilitated in vivo imaging of cellular structures and functions.
Conclusions:
- Transgenic mice are indispensable for elucidating gene function and disease mechanisms.
- Detailed protocols enable researchers to generate custom transgenic models for specific research questions.
- This chapter serves as a comprehensive guide for researchers utilizing transgenic mouse technology.