Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Knockout mouse models in pain research.

Andrée Dierich1, Brigitte L Kieffer

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.

Methods in Molecular Medicine
|May 8, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting Mu opioid receptor neurons of the habenula to limit naloxone aversion.

Biological psychiatry·2026
Same author

GPR88 localization to primary cilia in neurons is cell-type specific.

Life science alliance·2025
Same author

Incubation of social deficit during morphine abstinence in male mice using a novel unbiased and automatized method.

Frontiers in behavioral neuroscience·2025
Same author

The GPR88 Agonist RTI-122 Reduces Alcohol-Related Motivation and Consumption.

Addiction biology·2025
Same author

GPR88 localization to primary cilia in neurons is cell-type specific.

bioRxiv : the preprint server for biology·2025
Same author

Mu opioid receptor expression by nucleus accumbens inhibitory interneurons promotes affiliative social behavior.

bioRxiv : the preprint server for biology·2024
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Gene targeting in mice using homologous recombination creates knockout animals to study gene function in vivo. This chapter details methods for generating opioid receptor knockout mice and their phenotyping in pain assays.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Gene targeting in mice is crucial for understanding gene function in vivo.
  • Homologous recombination enables precise gene modification for studying complex biological processes.
  • Understanding nociceptive behaviors requires investigation of pain-related genes.

Purpose of the Study:

  • To provide detailed methodologies for creating gene-targeted knockout mice.
  • To exemplify the generation of mice lacking specific opioid receptor genes (mu, delta, kappa).
  • To outline protocols applicable to various gene-targeting projects.

Main Methods:

  • Protocols for embryonic stem (ES) cell production, maintenance, transfection, and selection.
  • Methods for blastocyst injection and subsequent generation of chimeric mice.

Related Experiment Videos

  • Strategies for constructing targeting vectors and genotyping ES cells and animals.

    • Main Results:

    • Successful generation of opioid receptor knockout mouse models.
    • Detailed protocols for ES cell manipulation and animal production are presented.
    • Examples of mouse phenotyping in pain behavioral assays are provided.

    Conclusions:

    • The described gene-targeting methods are broadly applicable to diverse gene knockout projects.
    • Opioid receptor knockout mice serve as valuable tools for pain research.
    • This chapter offers a comprehensive guide for researchers in gene targeting and pain mechanisms.