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Published on: June 1, 2012
[The effects of chitosan on different-derived fibroblasts]
Jing-De Zhang1, Xin Xing, Mei-qing Sun
1Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Objective:
To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.
Methods:
The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.
Results:
This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).
Conclusion:
The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.
