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Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Published on: November 8, 2016
Surface plasmon resonance study of carbohydrate-carbohydrate interaction between various gangliosides and
Kazunori Matsuura1, Ryuichi Oda, Hiromoto Kitakouji
1Department of Molecular Design, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
Abstract:
Carbohydrate-carbohydrate interactions between Gg3 trisaccharide-carrying polystyrene (PN(Gg3)) and monolayers of several glycosphingolipids (GSLs) were quantitatively investigated by surface plasmon resonance techniques. PN(Gg3) was adsorbed onto a GM3 monolayer strongly and specifically with an apparent affinity constant of K(a) = 2.5 x 10(6) M(-1), and the apparent affinity constants onto GSLs decreased in the following order: GM3 > LacCer > (KDN)GM3 approximately GlcCer > GM2 approximately GD3 approximately GM4 > GM1 approximately 2,6-isoGM3 > ceramide. These results suggest that PN(Gg3) recognizes not only some specified portions of GM3 but also the trisaccharide as a whole. On the other hand, PN(Lac) and PN(Cel) were bound to GSLs less strongly (K(a) approximately 10(4) M(-1)) and less selectively. The kinetic analysis revealed that the selectivity in the adsorption of PN(Gg3) onto the GM3 monolayer is dominated by the faster adsorption rate.
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