Related Experiment Video
Updated: Aug 24, 2026
Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
Published on: September 28, 2020
[Preparation and characterization of monoclonal antibodies against human laminin]
Min-jie Yao1, Han-chao Huang, Lian-xiu You
1Jilin Military Medical College Affiliated, Fourth Military Medical University of Chinese PLA, Jilin 132013, China.
Aim:
To prepare monoclonal antibody(mAb) against humen laminin(LN) and identify their properties.
Methods:
BALB/c mice were immunized with humen LN, and then mAb was prepared by hybridoma technique. The titres of mAbs in ascetic fluid and relative affinity of mAb were determined by indirect ELISA test. Ig subgroup, specificity and combine dot of mAb were analysed by ELISA.
Results:
4 hybridoma cell lines 2A3, 2C6, 3G7 and 4H2 secreting anti-humen LN mAb were obtained. Their Ig subgroup belonged to IgG1, light chains were type kappa, The titres of 4 mAbs in ascitic fluid were 3.6 x 10(4)-2.1 x 10(6). Relative affinity of mAb 2C6 was above 10(12) and relative affinity of other 3 mAbs 2A3, 3G7 and 4H2 was 10(6), 10(8) and 10(8). Epitope recognized by 2 mAb(2A3 and 4H2) differed from the that by 2C6 and 3G7 another.
Conclusion:
4 mAbs against humen LN has been prepared successfully and possess higher titer, specificity and relative affinity. Preparation of anti-human LN mAbs provide a useful reagent for further studying the role of LN in some diseases.
