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Published on: November 13, 2021
Mouse monoclonal IgA binds to the galectin-3/Mac-2 lectin from mouse macrophage cell lines
Rajko Reljic1, Carol Crawford, Stephen Challacombe
1Department of Oral Medicine and Pathology, Guy's Campus of King's College London, Guy's Tower Floor 28, Guy's Hospital, London SE1 9RT, UK.
Abstract:
The beta-galactoside-binding S-type lectin galectin-3/Mac-2, expressed among several other cell types on activated macrophages, is known to bind IgE, but not other Ig classes. We report in this paper that the single major constituent in a detergent lysate from the J774 mouse macrophage cell line bound to a mouse monoclonal IgA-affinity column. This fraction has been identified by mass spectrometry analysis as galectin-3. Binding of both mouse IgA and IgE to galectin-3 coated plates was inhibited by lactose and asialofetuin. Furthermore, three different monoclonal IgAs bound also to purified recombinant hamster galectin-3 coated plates in a concentration dependent manner. The potential functional significance of IgA binding to galectin-3 within macrophages and possibly other cell types is discussed.
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