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Low-density lipoprotein receptor-related protein interacts with MafB, a regulator of hindbrain development

Helle Heibroch Petersen1, Jan Hilpert, Christian Jacobsen

  • 1Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany. petersen@mdc-berlin.de

FEBS Letters
|May 12, 2004
PubMed

The intracellular domain (ICD) of the low-density lipoprotein receptor-related protein (LRP) functionally interacts with adaptor proteins both as an integral part of the receptor polypeptide and after proteolytic release. Identification of such adaptors has been difficult because the ICD is self-activating in conventional transcription factor-based yeast two-hybrid screens. We adopted an alternative screen for the ICD that depends on the activation of the Ras-signaling pathway and uncovered the transcription factor MafB as novel ICD interacting protein. MafB is a regulator of hindbrain segmentation and interacts with the ICD through a leucine zipper domain. The ICD co-localizes with MafB to the nucleus and negatively regulates its transcriptional activity, suggesting a possible role for LRP in brain development.

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