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Updated: Aug 24, 2026
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
Published on: October 23, 2014
Hyperoxia is not an essential condition for status epilepticus induced cell death in organotypic hippocampal slice
Jörn K Pomper1, Ulrike Hoffmann, Richard Kovács
1Johannes Müller Institute of Physiology, University Hospital Charité, Humboldt University Berlin, Tucholskystrasse 2, D-10117 Berlin, Germany. joern.pomper@gmx.de
Abstract:
The low Mg2+ model of epilepsy in organotypic hippocampal slice cultures is used to elucidate the mechanism underlying neuronal cell death following sustained epileptiform activity. However, the high oxygen tension of 95% widely used in this model is capable of inducing neuronal cell death by itself. Here we demonstrate that even under normoxic conditions 1h of epileptiform activity induced neuronal cell death as assessed by Propidium Iodide uptake. We conclude that hyperoxia is not essential for status epilepticus induced neuronal cell death in this model.
