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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
Published on: June 11, 2013
Automated sample preparation for the TRUGENE HIV-1 genotyping kit using the MagNA pure LC instrument
Jeffrey J Germer1, Jill N Vandenameele, P Shawn Mitchell
1Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Diagnostic Microbiology and Infectious Disease
|May 12, 2004
Abstract:
Use of the MagNA Pure LC Total Nucleic Acid Isolation Kit-Large Volume in conjunction with the TRUGENE HIV-1 Genotyping Kit yielded consistent, interpretable sequence data from 1-mL plasma preparations containing HIV-1 RNA concentrations of > or =400 copies/mL. This finding was confirmed in 18 of 24 low-titer clinical specimens.
