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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and

Satoru Fukiya1, Hiroshi Mizoguchi, Hideo Mori

  • 1Kyowa Hakko Branch, Japan Bioindustry Association in Tokyo Research Laboratories, Kyowa Hakko Kogyo, 3-6-6, Asahimachi, Machida-shi, Tokyo 194-8533, Japan.

FEMS Microbiology Letters
|May 12, 2004
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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