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Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes

Yoshihiro Honma1, Izumi Nakabayashi, Daisuke Tamaoki

  • 1Department of Biology, Toyama University.

Uchu Seibutsu Kagaku
|May 12, 2004
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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...