[Evaluation of the activity of monoclonal anti-A-, anti-B-antibodies]

The activity and specificity of monoclonal antibodies (MCA) anti-A and anti-B, which were presented for testing at the 4th International Workshop on Monoclonal Antibodies to Red Blood Cells and Related Antigens, Paris, 2001, were studied. First, the activity of antibodies with erythrocytes with good expression of antigens A and B (controls) was tested. Then, the activity of MCA with erythrocytes with a weak expression of erythrocyte antigens was tested and the reaction was, after that, estimated in percent versus the controls. According to the results, the activity of interaction of anti-B antibodies with the phenotype A2 erythrocytes was lower versus such activity with the phenotype A1 erythrocytes. It is noteworthy, that all samples of antibodies, except for series 5, 6 and 10, were more active with the phenotype A2 erythrocytes versus the phenotype A2B erythrocytes. All tested samples of anti-B-antibodies possessed a high activity (within a range of 85-100%) and were effective in the detection of antigen B. The activity of MCA anti-AB with erythrocytes A2 was lower versus such activity with erythrocytes A and B and made 45-85%. MCAs of anti-A and of anti-B significantly differed by their ability to interact with erythrocytes A2. This may generate problems in using the above antibodies for making the typing reagents to be applied in routine blood group tests.