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Updated: Aug 17, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Phylogenetic analysis of Verticillium species based on nuclear and mitochondrial sequences
Jan Fahleson1, Qiong Hu, Christina Dixelius
1Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, (SLU), P.O. Box 7080, 750 07, Uppsala, Sweden. Jan.Fahleson@vbsg.slu.se
Abstract:
Three different genes were sequenced from isolates of five plant-pathogenic Verticillium species, Verticillium albo-atrum, Verticillium dahliae, Verticillium longisporum, Verticillium nigrescens, and Verticillium tricorpus. The sequences covered parts of the mitochondrial cytochrome b gene ( cob), the mitochondrial small subunit rRNA gene ( rns) and the nuclear ITS2 region. When the sequences were combined, the five species clustered in five monophyletic groups, with V. nigrescens distantly related to the other species while V. tricorpus displayed a somewhat closer relationship to the three remaining species. V. albo-atrum, V. dahliae and V. longisporum were found to be very similar to each other, with V. albo-atrum and V. longisporum displaying the closest relationship. The species affiliation of V. longisporum is discussed.
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