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Updated: Aug 24, 2026

Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
[The antitussive and de-sputum essential substance from the root of Gerbera piloselloides and its analytical
Xiao-jiang Tang1, Hua-rong Huang, Tie-zheng Fang
1Zhongshan University, Guangdong Provincial Center for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong, China. Chao-zhu@163.com
Objective:
To study the chemical constituents of the essential substance from the root of Gerbera piloselloides and its antitussive and de-sputum effects.
Method:
The essential substance (G4) was extracted from the root by alcohol and ethyl acetate, then it was separated by silica gel column eluted by the mixture of ethyl acetate and petroleum ether (5:95). Its chemical components were separated and identified by GC-MS. Its antitussive and de-sputum effect was tested by mice.
Result:
4 main peaks were separated and identified by GS-MS. They are beta-caryophyllene (15.160%), caryophyllene oxide (21.140%), aristolenepoxide (2.673%) and 6-acetyl-2,2-dimethyl-8(3-methyl-2-butenyl)-2H-chromoene (60.077%) respectively. Its antitussive and de-sputum effect was prominent when the mice was given G4 2,000 mg.kg-1 ig.
Conclusion:
Itis the first time that the antitussive and de-sputum essential substance was separated from the root of Gerbera piloselloides and its main compositions were analyzed.