[Matrine effects on JM cells by inhibiting proliferation and inducing apoptosis]
Ji-liang Feng1, Gao-sheng Huang, Yong-qing Zhang
1Molecular Oncology Laboratory, Department of Pathology, Fourth Military Medical University, Xi'an 710032, Shanxi, China. jilangfeng@hotmail.com
Objective:
To study effects of matrine on JM cell strain.
Method:
Morphologic changes were observed under light microscope with Wright-Giemsa staining, fluorescence microscope with Hoechst 33,258 staining and electron microscope. Alteration of cell cycle of different dose treating groups at the fourth day and 0.8 mg.mL-1 treatment group at the first, second, third, fourth day was analyzed by Flow cytometry. DNA ladder was detected with gel electrophoresis.
Result:
From the third day after treatment of matrine, typical apoptosis features of cells were observed under light microscope and electron microscope in all test groups, and the features were more prominent with the time prolonging. At fourth day, flow cytometry analysis showed that there were sub-G1 peaks in all groups. From 0.1, 0.2, 0.4, 0.6 to 0.8 g.L-1 treatment groups, the rate of apoptotic cells to total cells were 3.1%, 2. 5%, 13.3%, 40.4%, 48.6%, respectively, and what in the control group was 1.4%; the rate of S phase cells to total cells was 28.9%, 26.1%, 27.7%, 0.9%, 14.2%, what in the control group was 30.4%; the rate of G1 phase cells to total cells was 63. 2%, 67.5%, 68.1%, 75.2%, 83.6%, what in the control group was 41.8%; From the first, second, third to fourth day, the rate of apoptotic cells to total cells of 0.8 mg.mL-1 treatment group were 3.0%, 3.7%, 9.1%, 48.6%, respectively; the rate of S phase cells to total cells was 28.6%, 17.5%, 19.1%, 14.2%; the rate of G1 phase cells to total cells were 45.5%, 77.3%, 77.2%, 83.6%. Gel electrophoresis displayed "DNA ladder" in 0.4, 0.6, 0.8 g.L-1 groups, while 0.1 and 0.2 g.L-1 groups didn't show such result.
Conclusion:
Matrine can repress DNA synthesis and arrest JM cell strain at G1 phase, sequentially inhibiting the proliferation of the cell. Besides, this alkaloid can induce the apoptosis of JM cells.
Insights
Matrine treatment induces apoptosis and inhibits proliferation in JM cells by repressing DNA synthesis and arresting the cell cycle at the G1 phase. These findings highlight matrine
Area of Science:
- Pharmacology
- Cell Biology
- Biochemistry
Background:
- Matrine is a natural alkaloid with potential therapeutic properties.
- Understanding its effects on specific cell lines is crucial for drug development.
Purpose of the Study:
- To investigate the effects of matrine on the JM cell strain.
- To elucidate the mechanisms underlying matrine's action on cell proliferation and survival.
Main Methods:
- Morphological analysis using light, fluorescence, and electron microscopy.
- Cell cycle analysis via flow cytometry.
- DNA fragmentation detection using gel electrophoresis.
Main Results:
- Matrine treatment led to typical apoptosis features in JM cells.
- Flow cytometry revealed sub-G1 peaks and increased G1 phase arrest with higher matrine concentrations.
- DNA laddering, indicative of apoptosis, was observed at higher matrine doses.
Conclusions:
- Matrine inhibits JM cell proliferation by repressing DNA synthesis and inducing G1 phase arrest.
- Matrine effectively induces apoptosis in JM cells.
- These results suggest matrine's potential as an anti-proliferative and apoptotic agent.
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