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[Study on preparation process and analytical methods of ESAC from Ganoderma lucidum]
Shu-ming Huang1, Xin-lin Yang, Bang-wu Wang
1School of Life Sciences and Technology, Beijing Institute of Technology, Beijing 100081, China.
Summary
This study details a simple procedure for isolating ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum. A reliable method for quantifying ESAC in G. lucidum products was also established.
Area of Science:
- Pharmacognosy
- Natural Product Chemistry
Background:
- Ganoderma lucidum is a medicinal mushroom with various bioactive compounds.
- Standardized methods are needed for quality control of G. lucidum products.
Purpose of the Study:
- To develop a procedure for separating ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum.
- To establish a quantitative analysis method for ESAC in G. lucidum.
Main Methods:
- ESAC was extracted from G. lucidum using ethanol, saturated NaHCO3, and chloroform.
- Quantitative analysis involved a color reaction with H2SO4.
Main Results:
- Optimal separation conditions were determined for a 10g scale, including material-to-ethanol ratio (1:15) and extraction times.
- Specific parameters for ESAC quantification were established, including sample weight (1g), reagent concentration (50% H2SO4 in ethanol), and wavelength (490 nm).
Conclusions:
- The developed ESAC preparation procedure is simple and effective.
- The quantitative method is suitable for analyzing G. lucidum related products.