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Updated: Aug 24, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
Published on: May 21, 2021
High-throughput preparative process utilizing three complementary chromatographic purification technologies
Manuel Ventura1, William Farrell, Christine Aurigemma
1Pfizer Global R&D-La Jolla, 10770 Science Center Drive, San Diego, CA 92121, USA. manuel.ventura@pfizer.com
Abstract:
A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded.
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