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Cloning and sequencing of hepatitis B virus pre-S and S gene regions
1Department of Biochemistry, University of Zimbabwe, Mount Pleasant, Harare.
Scandinavian Journal of Immunology. Supplement
|January 1, 1992
Abstract:
The polymerase chain reaction (PCR) was used to amplify the pre-S1, pre-S2 and S gene regions of hepatitis B virus (HBV). Sera from three different patients were used as the source of HBV DNA. The resulting 1.2-kb amplification product was cloned into the plasmid pIBI30. Restriction enzyme analysis revealed that there are two BamHI sites located about 300 bp apart within the S gene. DNA sequencing revealed a greatest homology to the HBVadw subtype.