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Related Experiment Videos

Density-dependent selection in vesicular stomatitis virus.

Isabel S Novella1, Daniel D Reissig, Claus O Wilke

  • 1Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Ave., Toledo, OH 43614, USA. isabel@mco.edu

Journal of Virology
|May 14, 2004
PubMed
Summary

Complementation significantly impacts the fitness of deleterious viral mutants, like monoclonal antibody-resistant mutant (MARM) N, during vesicular stomatitis virus coinfections. Viral fitness depends on the multiplicity of infection, not initial mutant frequencies.

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Area of Science:

  • Virology
  • Evolutionary Biology
  • Molecular Biology

Background:

  • Complementation, the phenomenon where different mutant viruses can provide functions missing in each other, is crucial for understanding viral evolution.
  • Deleterious mutations can persist in viral populations through mechanisms like complementation.

Purpose of the Study:

  • To investigate the effect of complementation on the relative fitness of a deleterious mutant (MARM N) of vesicular stomatitis virus (VSV) in competition with its wild-type ancestor.
  • To determine how multiplicity of infection (MOI) and initial viral frequencies influence this fitness.

Main Methods:

  • Competitions between MARM N and wild-type VSV were conducted at varying MOIs and initial population ratios.
  • A mathematical model was developed assuming shared gene products during coinfection.

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Main Results:

  • The relative fitness of MARM N was highly sensitive to changes in MOI, indicating a strong dependence on complementation levels.
  • Initial frequencies of the wild type and mutant had minimal impact on MARM N's relative fitness.
  • Experimental results showed good agreement with the mathematical model's predictions.

Conclusions:

  • Complementation plays a significant role in determining the relative fitness of viral mutants.
  • VSV wild-type and MARM N freely share gene products during coinfection, supporting the developed model.
  • The degree of complementation, modulated by MOI, is a key factor in the evolutionary dynamics of viral mutants.