Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methyl selenium metabolites decrease prostate-specific antigen expression by inducing protein degradation and

Sung Dae Cho1, Cheng Jiang, Barbara Malewicz

  • 1Hormel Institute, University of Minnesota, Austin 55912, USA.

Molecular Cancer Therapeutics
|May 14, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The epidemiology and clinical features of post-exposure prophylaxis for rabies: A retrospective study of 9772 cases.

One health (Amsterdam, Netherlands)·2024
Same author

Genetic polymorphisms, biomarkers and signaling pathways associated with septic shock: from diagnosis to therapeutic targets.

Burns & trauma·2024
Same author

MixFormer: End-to-End Tracking With Iterative Mixed Attention.

IEEE transactions on pattern analysis and machine intelligence·2024
Same author

Multi-omics analysis reveals the association between specific solute carrier proteins gene expression patterns and the immune suppressive microenvironment in glioma.

Journal of cellular and molecular medicine·2024
Same author

PagPXYs improve drought tolerance by regulating reactive oxygen species homeostasis in the cambium of Populus alba × P. glandulosa.

Plant science : an international journal of experimental plant biology·2024
Same author

Type I [4σ+4π] versus [4σ+4π-1] Cycloaddition To Access Medium-Sized Carbocycles and Discovery of a Liver X Receptor β-Selective Ligand.

Angewandte Chemie (International ed. in English)·2024

Methylselenol and methylseleninic acid (MSeA) inhibit prostate-specific antigen (PSA) expression and secretion in prostate cancer cells. These selenium compounds reduce PSA protein levels and suppress PSA gene transcription, offering insights into selenium

Area of Science:

  • Biochemistry and Molecular Biology
  • Oncology
  • Nutritional Science

Background:

  • Prostate-specific antigen (PSA) is a critical biomarker for prostate cancer diagnosis, treatment monitoring, and recurrence detection.
  • Selenium is being investigated for prostate cancer chemoprevention, with PSA serving as a key response biomarker in clinical trials.
  • Understanding selenium metabolite effects on PSA kinetics is vital for interpreting chemoprevention trial outcomes.

Purpose of the Study:

  • To investigate whether methylselenol or methylseleninic acid (MSeA), putative active selenium metabolites, inhibit PSA expression in prostate cancer cells.
  • To elucidate the mechanisms by which MSeA affects PSA turnover, secretion, and gene expression.
  • To compare the effects of MSeA and methylselenol with other selenium compounds like sodium selenite and selenomethionine.

Related Experiment Videos

Main Methods:

  • Exposure of androgen-responsive LNCaP prostate cancer cells to sub-apoptotic concentrations of MSeA, methylselenol, sodium selenite, and selenomethionine.
  • Measurement of PSA protein expression, secretion, and mRNA levels.
  • Analysis of PSA protein degradation pathways using lysosomal and proteasomal inhibitors.
  • Assessment of androgen receptor (AR) protein expression and AR-stimulated PSA promoter activity.

Main Results:

  • MSeA and methylselenol significantly inhibited PSA protein expression and secretion, while sodium selenite and selenomethionine showed no inhibitory effect.
  • Inhibition of PSA was observed as early as 3 hours and required a sustained MSeA threshold.
  • MSeA induced rapid PSA protein degradation, primarily via lysosomal pathways, and reduced PSA mRNA levels.
  • MSeA treatment also down-regulated androgen receptor protein and inhibited androgen-stimulated PSA promoter transcription.

Conclusions:

  • Methylselenol and MSeA specifically inhibit PSA expression in prostate cancer cells through dual mechanisms: enhanced PSA protein degradation and suppressed transcriptional activity.
  • These findings provide mechanistic insights into the potential prostate cancer chemopreventive effects of selenium, particularly its active metabolites.
  • The differential effects of selenium compounds highlight the importance of metabolite-specific actions in selenium's biological activities.