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Updated: Aug 24, 2026
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
[Significance of stathmin gene overexpression in osteosarcoma cells]
Hui-Zhong Zhang1, Ping Gao, Lu Yan
1Center of Clinical Research, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710038, PR China. huizhong@public.xa.sn.cn
Background & Objective:
Stathmin, a signal transduction regulatory factor, plays a crucial role in cell division and malignant tumor development. This study was designed to analyze stathmin gene expression in the tissue of osteosarcoma, and to explore the growth inhibition by blocking stathmin expression with Stathmin antisense oligodeoxynucleotide (ASODN) on cultured human osteosarcoma cell line with stathmin gene overexpression.
Methods:
Reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization methods were used to determine the expression of stathmin in two human osteosarcoma cell lines and 45 osteosarcoma tissue specimens. Using stathmin gene overexpression in human osteosarcoma cell line SOSP-9607 as target cell and stathmin ASODN as gene expression blocking agent, cell growth inhibition of stathmin ASODN was determined by MTT method, and cell growth and mitotic character was analyzed by flow cytometry.
Results:
Both osteosarcoma cell lines of SOSP-9607 and SOSP-9901 showed stathmin gene overexpression by RT-PCR method and 24 of 45 osteosarcoma specimens were stathmin positive by in situ hybridization, while there were only 2 in 10 normal tissues with mild positive signal. There was significant difference in stathmin gene expression between human osteosarcoma tissues and normal tissues (P< 0.05). The growth of osteosarcoma cells were suppressed by ASODN (P< 0.05,P< 0.01). The cell cycle of SOSP-9607 was blocked in metaphase and cell apoptosis was found.
Conclusion:
Stathmin gene displays high level expression in osteosarcoma, which may become a new target for the bio-treatment for osteosarcoma.
Insights
Stathmin gene is overexpressed in osteosarcoma tissues and cell lines. Blocking stathmin expression with antisense oligodeoxynucleotide (ASODN) inhibited osteosarcoma cell growth and induced apoptosis, suggesting stathmin as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Context:
- Stathmin is a key regulator of cell division and implicated in cancer development.
- Osteosarcoma is a primary bone malignancy with significant morbidity.
- Understanding stathmin's role in osteosarcoma is crucial for developing targeted therapies.
Purpose:
- To investigate stathmin gene expression levels in osteosarcoma tissues and cell lines.
- To evaluate the efficacy of stathmin antisense oligodeoxynucleotide (ASODN) in inhibiting osteosarcoma cell growth.
- To explore the impact of stathmin inhibition on cell cycle progression and apoptosis.
Summary:
- Stathmin gene overexpression was confirmed in osteosarcoma cell lines and patient tissues compared to normal controls.
- Treatment with stathmin ASODN significantly suppressed osteosarcoma cell proliferation.
- Flow cytometry analysis revealed cell cycle arrest at metaphase and induced apoptosis in stathmin-targeted cells.
Impact:
- Stathmin gene expression is a potential biomarker for osteosarcoma.
- Stathmin ASODN demonstrates therapeutic potential for osteosarcoma treatment.
- This study identifies stathmin as a promising molecular target for novel osteosarcoma bio-therapies.
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