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Related Experiment Videos

Urea biosensor based on PANi(urease)-Nafion/Au composite electrode.

Yung-Chien Luo1, Jing-Shan Do

  • 1Department of Chemical Engineering, Tunghai University, Taichung 40704, Taiwan, ROC.

Biosensors & Bioelectronics
|May 15, 2004
PubMed
Summary

This study developed a novel polyaniline-Nafion composite electrode for detecting urea. The optimized electrode demonstrated high sensitivity and a low detection limit for urea sensing.

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Biosensors

Background:

  • Polyaniline (PANi)-Nafion composite films offer unique electrochemical properties.
  • Developing sensitive and selective biosensors for urea detection is crucial for various applications.

Purpose of the Study:

  • To prepare and characterize a PANi-Nafion composite film on a ceramic plate using cyclic voltammetry (CV).
  • To immobilize urease onto the composite film for urea detection.
  • To evaluate the performance of the developed urea sensor.

Main Methods:

  • Preparation of PANi-Nafion composite film via CV with varying cycle numbers.
  • Electrochemical characterization of the composite electrode.
  • Immobilization of urease using electrochemical and casting methods.

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  • Detection of urea concentration in buffer solutions.

    • Main Results:

    • The composite electrode prepared with 5 CV cycles showed the highest cathodic peak current in NH4Cl buffer.
    • Cathodic peak current decreased with increasing CV cycles.
    • The casting immobilization method yielded higher sensitivity compared to electrochemical immobilization.
    • The urea sensor achieved a sensitivity of 0.7 µA (mg dL⁻¹)⁻¹cm⁻² and a detection limit of 0.3 mg dL⁻¹.

    Conclusions:

    • The PANi-Nafion composite film prepared by CV is a promising platform for biosensor development.
    • Urease immobilization via casting enhances sensor performance.
    • The developed urea sensor exhibits excellent sensitivity and a low detection limit.