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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Published on: May 5, 2014
Sensitivity of human adenoviruses to different groups of chemical biocides
A Sauerbrei1, K Sehr, A Brandstädt
1Institute of Virology and Antiviral Therapy, Friedrich-Schiller University, Jena, Germany. andreas.sauerbrei@med.uni-jena.de
Abstract:
As it is no longer readily permissible to propagate polioviruses, adenoviruses will probably become more frequently used for virucidal testing of broad-spectrum disinfectants. We tested prototype strains of different adenovirus serotypes belonging to the subgenera C and D for sensitivity to liposomal povidone-iodine (PVP-I), peracetic acid (PAA) and formaldehyde. Virucidal assays were performed as suspension tests in accordance with the German guidelines for testing the effectiveness of chemical disinfectants against viruses. The sensitivity of the adenovirus serotypes to test disinfectants varied greatly. Prototype strains of adenovirus 5 and 44 statistically selected as 'little sensitive' to PVP-I, PAA and formaldehyde were found to be the most resistant. These might be suitable as model viruses for testing the broad-spectrum virucidal activity of disinfectants and might even replace the resistant poliovirus type 1. The main reason for the different chemical sensitivity of adenovirus serotypes may be the composition of the viral capsid proteins. Alternatively a different chemical inactivation of the viral genome could be involved.
Insights
Adenoviruses are proposed as replacements for polioviruses in disinfectant testing. Certain adenovirus strains, like types 5 and 44, show resistance making them ideal models for broad-spectrum virucidal activity assessments.
Area of Science:
- Virology
- Microbiology
- Disinfectant Efficacy Testing
Background:
- Polioviruses are no longer readily propagated for virucidal testing.
- Adenoviruses are emerging as potential replacements for poliovirus in disinfectant efficacy studies.
- Broad-spectrum disinfectant testing requires robust viral models.
Purpose of the Study:
- To evaluate the sensitivity of different adenovirus serotypes to common disinfectants.
- To identify adenovirus strains suitable as model viruses for broad-spectrum disinfectant testing.
- To compare adenovirus resistance profiles with poliovirus resistance.
Main Methods:
- Suspension tests were conducted according to German guidelines for virucidal assays.
- Prototype strains of adenovirus subgenera C and D were tested.
- Sensitivity to liposomal povidone-iodine (PVP-I), peracetic acid (PAA), and formaldehyde was assessed.
Main Results:
- Adenovirus serotype sensitivity to disinfectants varied significantly.
- Adenovirus types 5 and 44 demonstrated the highest resistance to PVP-I, PAA, and formaldehyde.
- These resistant strains could serve as effective model viruses, potentially replacing poliovirus type 1.
Conclusions:
- Adenovirus serotypes exhibit differential sensitivity to chemical disinfectants.
- Adenovirus types 5 and 44 are promising candidates for modeling broad-spectrum virucidal activity.
- Variations in viral capsid protein composition may explain differences in disinfectant sensitivity.
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