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Related Experiment Videos

Characterization and tissue-specific expression of human LRIG2.

Camilla Holmlund1, Jonas Nilsson, Dongsheng Guo

  • 1Department of Radiation Sciences, Oncology, Umeå University, SE-90187, Umeå, Sweden.

Gene
|May 18, 2004
PubMed
Summary

Researchers identified and characterized the LRIG2 gene, a novel glycoprotein. Its mRNA is expressed in all human tissues, with highest levels in skin, uterus, and brain.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Protein Biochemistry

Background:

  • The LRIG1 gene was previously identified as a human integral membrane protein.
  • LRIG1 shares domain organization with Drosophila Kekkon-1, an EGF-receptor antagonist.
  • The study aimed to identify and characterize additional members of the human LRIG gene family.

Purpose of the Study:

  • To identify and characterize the novel human LRIG2 gene and its protein product.
  • To determine the tissue distribution and localization of LRIG2.
  • To investigate the post-translational modifications and cellular location of LRIG2.

Main Methods:

  • Gene identification and sequencing
  • Chromosomal localization
  • Northern blotting and RT-PCR for transcript analysis

Related Experiment Videos

  • Quantitative real-time RT-PCR for expression levels
  • Immunoblotting and N-glycosidase F treatment for protein characterization
  • Cell surface biotinylation and confocal microscopy for localization

    • Main Results:

    • The LRIG2 gene was identified, localized to chromosome 1p13, and encodes a 1065-amino acid protein.
    • LRIG2 shares 47% amino acid identity with LRIG1 and exhibits similar domain organization.
    • LRIG2 is a glycoprotein expressed in all analyzed tissues, with prominent expression in skin, uterus, ovary, kidney, brain, and others.
    • LRIG2 is detected at the cell surface and in the cytoplasm.

    Conclusions:

    • LRIG2 is a novel glycoprotein encoded by a gene on human chromosome 1p13.
    • LRIG2 mRNA is ubiquitously expressed across all human tissues analyzed.
    • The protein is expressed at the cell surface and within the cytoplasm.