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Updated: Jul 28, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Published on: February 3, 2013
Ultrastructural cytochemical analyses of nuclear functional architecture
1Centre of Electron Microscopy University of Lausanne, Lausanne, Switzerland. sfakan@cme.unil.ch
Abstract:
For many years, ultrastructural cytochemistry has been a major tool for investigating the relationships between the structure and the functions of the cell nucleus. This article shortly reviews the contributions of transmission electron microscopy to the in situ studies of intranuclear distribution of chromatin domains and of essential nuclear functions, such as DNA replication, transcription and pre-mRNA processing. It attempts to analyse the role of different nuclear structural domains in nuclear functions, as well as further directions in this important field of research.
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