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B-cell development and pre-B-1 cell plasticity in vitro

Antonius G Rolink1

  • 1Division of Molecular Immunology, Pharmazentrum, University of Basel, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2004
PubMed

B-lymphopoiesis in vivo is a very complex process that is influenced by cooperation between cells, cytokines, and other receptor-ligand interactions, which developmentally occur at different cellular stages. Various in vitro models have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test the plasticity of Pax-5-deficient pre-B-1 cells are also outlined.

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