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Microbioreactor arrays with parametric control for high-throughput experimentation.

Michel M Maharbiz1, William J Holtz, Roger T Howe

  • 1Berkeley Sensor & Actuator Center (BSAC), Dept. of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, California 94720, USA.

Biotechnology and Bioengineering
|May 19, 2004
PubMed
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This study introduces a scalable microbioreactor array for high-throughput cell cultivation, enabling precise control over parameters like temperature and gas levels. This technology enhances reproducibility in cell culture experiments.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microfluidics

Background:

  • High-throughput cell cultivation requires precise control over environmental parameters.
  • Existing methods for controlling gas and pH in microscale cultures can be complex and lack scalability.

Purpose of the Study:

  • To demonstrate a scalable array technology for parametric control of high-throughput cell cultivations.
  • To integrate commercial printed circuit board (PCB) technology, sensors, and electrochemical gas generation for microbioreactors.

Main Methods:

  • Developed an array of eight 250 microl microbioreactors using PCB technology.
  • Integrated closed-loop temperature control, electrochemical feed gas generation, and optical density monitoring.
  • Incorporated a commercial ISFET chip for continuous pH monitoring and an electrochemical dosing system for gas delivery.

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Main Results:

  • Demonstrated independent parametric control for each microbioreactor in the array.
  • Presented growth data for Escherichia coli under varying microaerobic conditions using electrochemically generated oxygen.
  • Showcased reproducible gas delivery and pH control using electrochemical systems.

Conclusions:

  • The developed array technology offers a scalable and reproducible platform for high-throughput cell cultivation.
  • Integration of commercial components and electrochemical systems simplifies and enhances control over microreactor environments.
  • This technology facilitates precise manipulation of microaerobic conditions for cell growth studies.