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Expression pattern of apolipoprotein M during mouse and human embryogenesis
Xiao-Ying Zhang1, Guo-Qing Jiao, Maria Hurtig
1Laboratory of Molecular Medicine, The Third Affiliated Hospital, SuZhou University, ChangZhou, China.
Acta Histochemica
|May 19, 2004
Summary
Apolipoprotein M (apoM) expression is significant during mammalian embryogenesis, primarily in the liver and kidney. This study maps apoM gene expression patterns in developing mouse and human embryos.
Area of Science:
- Biochemistry
- Developmental Biology
- Genetics
Background:
- Apolipoprotein M (apoM) is a protein found in lipoproteins like HDL, LDL, and TGRLP.
- The physiological roles of apoM remain largely unknown.
- The apoM gene is conserved across mammalian species.
Purpose of the Study:
- To investigate the expression patterns of apolipoprotein M during mouse and human embryogenesis.
- To identify the specific tissues and developmental stages where apoM is expressed.
Main Methods:
- Analysis of apoM transcript detection in mouse embryos from day 7.5 to 18.5.
- In situ hybridization to locate apoM-positive cells in mouse embryos.
- mRNA array analysis of human fetal tissues to determine apoM expression.
Main Results:
- ApoM transcripts were detected throughout mouse embryogenesis, with dynamic expression levels peaking at specific stages.
- In mouse embryos, apoM expression was primarily observed in the liver and later in the kidney.
- In human embryos, apoM was predominantly expressed in the liver and kidney, with lower levels in the small intestine, stomach, and skeletal muscle.
Conclusions:
- Apolipoprotein M exhibits distinct spatiotemporal expression patterns during mammalian embryogenesis.
- The expression of apoM in key organs like the liver and kidney suggests a potential role in embryonic development.
- Further research is warranted to elucidate the specific functions of apoM during development.