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Related Experiment Videos

Genetic deletion of ghrelin does not decrease food intake but influences metabolic fuel preference.

Katherine E Wortley1, Keith D Anderson, Karen Garcia

  • 1Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 19, 2004
PubMed
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Endogenous ghrelin does not appear essential for regulating appetite or body weight. However, ghrelin-deficient mice show altered metabolic substrate utilization, particularly with high-fat diets.

Area of Science:

  • Endocrinology
  • Neuroscience
  • Metabolism

Background:

  • Ghrelin, a growth hormone secretogogue, stimulates food intake and adiposity.
  • Its role in appetite regulation suggests potential therapeutic targets for obesity.
  • Previous studies focused on exogenous ghrelin's effects, necessitating investigation into endogenous functions.

Purpose of the Study:

  • To investigate the role of endogenous ghrelin in appetite and body weight regulation.
  • To characterize ghrelin-deficient mice (ghrl(-/-)) and their physiological responses.
  • To determine ghrelin's influence on metabolic substrate utilization.

Main Methods:

  • Generation of ghrelin-deficient (ghrl(-/-)) mice by replacing the ghrelin gene with a lacZ reporter.
  • Assessment of viability, growth rates, and spontaneous food intake patterns.

Related Experiment Videos

  • Analysis of basal neuropeptide levels and fasting-induced hyperphagia.
  • Evaluation of metabolic substrate utilization in ghrelin-deficient mice.

    • Main Results:

    • Ghrelin-deficient mice were viable with normal growth and food intake.
    • No impairment in reflexive hyperphagia after fasting was observed.
    • Endogenous ghrelin is not essential for appetite regulation, playing at most a redundant role.
    • Ghrelin-deficient mice exhibited altered metabolic substrate utilization, favoring carbohydrates over fats, especially during high-fat intake.

    Conclusions:

    • Endogenous ghrelin is not a primary regulator of appetite or body weight.
    • Ghrelin plays a significant role in determining metabolic substrate preference.
    • These findings suggest ghrelin's involvement in metabolic flexibility rather than appetite control.