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Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases
Paul F van Swieten1, Rene Maehr, Adrianus M C H van den Nieuwendijk
1Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
Bioorganic & Medicinal Chemistry Letters
|May 20, 2004
Abstract:
Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.