Related Experiment Video
Updated: Aug 24, 2026

05:04
Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm
Published on: October 13, 2023
Electron microscopic cytochemistry of adenylyl cyclase activity in mouse spermatozoa
Lavinia Liguori1, Maria Grazia Rambotti, Ilaria Bellezza
1Dipartimento di Scienze Biochimiche Biotecnologie Molecolari, Sezione Biochimica Cellulare, Università di Perugia, Perugia, Italia.
Abstract:
We investigated adenylyl cyclase activity of mouse spermatozoa by electron microscopic cytochemistry. Subcellular localization of enzyme activity was determined in the presence and absence of bicarbonate ions. Results confirm the existence in sperm of a bicarbonate-regulated adenylyl cyclase, which suggests microdomain signaling.

