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A piezoelectric immunobiosensor for atrazine in drinking water
G G Guilbault1, B Hock, R Schmid
1Department of Botany, Technische Universität München-Weihenstephan, Freising, Germany.
Biosensors & Bioelectronics
|January 1, 1992
Summary
A new piezoelectric crystal immunobiosensor accurately detects atrazine herbicides in drinking water. This reusable sensor offers sensitive and reliable measurements for water quality monitoring.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Biotechnology
Background:
- Atrazine is a widely used herbicide that can contaminate drinking water sources.
- Accurate and sensitive detection methods are crucial for monitoring atrazine levels in water.
Purpose of the Study:
- To develop a novel piezoelectric crystal immunobiosensor for the quantitative analysis of atrazine in drinking water.
Main Methods:
- Utilized a 10 MHz piezoelectric crystal coated with protein A and atrazine antibodies.
- Immobilized polyclonal antibodies from sheep onto the gold electrode of the crystal.
- Employed the sensor for detecting atrazine concentrations in water samples.
Main Results:
- The immunobiosensor demonstrated reliable detection of atrazine within a range of 0.03-100 µg/L (ppb).
- Achieved a relative standard deviation of approximately +/- 8% for atrazine determinations.
- The sensor proved to be reversible and reusable for 8-9 assays.
Conclusions:
- The developed piezoelectric crystal immunobiosensor is a viable tool for sensitive and accurate atrazine detection in drinking water.
- The sensor's reusability and sensitivity make it a cost-effective option for water quality assessment.
- This technology contributes to effective environmental monitoring and public health protection.